Part:BBa_K563055:Design

pSB1A11, reconstructed from pSB1A2 with NcoI, XhoI, BamHI and PstI

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal PstI site found at 167
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal PstI site found at 167
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BamHI site found at 153
  Illegal XhoI site found at 133
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal PstI site found at 167
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal PstI site found at 167
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1248
  

Design Notes

In order to make our mutation work easier, we separate Tor2 into three parts. so we add fout certain restriction sites into the vector to make the connection easier to conduct.

Source

pSB1A2 is from BioBrick